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The Knife-Edge Scanning Microscope (KESM) was invented and patented in the late 1990s by Bruce McCormick at Texas A&M University. [1] The microscope is intended to produce high-resolution data sets in order to reconstruct 3D cellular structures .
A digital microscope is a variation of a traditional optical microscope that uses optics and a digital camera to output an image to a monitor, sometimes by means of software running on a computer. A digital microscope often has its own in-built LED light source, and differs from an optical microscope in that there is no provision to observe the ...
The resolution at that time was limited to 10 µm laterally and 26 µm longitudinally but at a sample size in the millimeter range. The orthogonal-plane fluorescence optical sectioning microscope used a simple cylindrical lens for illumination. Further development and improvement of the selective plane illumination microscope started in 2004. [5]
Without oil, light waves reflect off the slide specimen through the glass cover slip, through the air, and into the microscope lens (see the colored figure to the right). Unless a wave comes out at a 90-degree angle, it bends when it hits a new substance, the amount of bend depending on the angle. This distorts the image.
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
A petrographic microscope is a type of optical microscope used to identify rocks and minerals in thin sections. The microscope is used in optical mineralogy and petrography, a branch of petrology which focuses on detailed descriptions of rocks. The method includes aspects of polarized light microscopy (PLM).
A microscope slide (top) and a cover slip (bottom) A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope. Typically the object is mounted (secured) on the slide, and then both are inserted together in the microscope for viewing. This ...
Royal Raymond Rife (May 16, 1888 – August 5, 1971) [1] was an American inventor and early exponent of high-magnification time-lapse cine-micrography. [2] [3]Rife is known for his microscopes, which he claimed could observe live microorganisms with a magnification considered impossible for his time, and for an "oscillating beam ray" invention, which he thought could treat various ailments by ...