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Serum protein electrophoresis (SPEP or SPE) is a laboratory test that examines specific proteins in the blood called globulins. [1] The most common indications for a serum protein electrophoresis test are to diagnose or monitor multiple myeloma , a monoclonal gammopathy of uncertain significance (MGUS), or further investigate a discrepancy ...
In medicine, protein electrophoresis is a method of analysing the proteins mainly in blood serum. Before the widespread use of gel electrophoresis , protein electrophoresis was performed as free-flow electrophoresis (on paper) or as immunoelectrophoresis.
Normal total protein levels are not sufficient to rule out multiple myeloma or other malignant paraproteinemias, but they may also be the cause of moderate-to-marked hyperproteinemia. To determine the reason behind the elevated serum total protein, a serum protein electrophoresis should be carried out.
Researchers have identified protein signatures in blood tests that can be used to detect or predict up to 67 diseases, using UK Biobank data in a cohort of nearly 42,000 participants.
Counter-immunoelectrophoresis and its modification. In comparison to other conventional methods of diagnosis e.g. for viral infection testing, counter-immunoelectrophoresis is a highly specific, simple, and speedy method that does not require sophisticated, expensive tools, input materials, or long-term capacity building.
The serum free light-chain assay in combination with serum protein electrophoresis and serum immunofixation electrophoresis is sufficient to screen for pathological monoclonal plasmaproliferative disorders other than AL amyloidosis which requires all the serum tests as well as 24 h urine immunofixation electrophoresis.
There is the total protein test or the serum protein electrophoresis which measures the level of each protein in a patient's blood. The serum protein electrophoresis test focuses more on the immune system and if it is working properly and it measures the levels of several types of globulins or proteins in the blood. [6]
Pipetting anti-immunoglobulins to immunofixation panel. The panel simultaneously tests 4 patients (one in each quadrant). Each patient has 6 electrophoresis panels: The left one is a conventional serum protein electrophoresis. The remainder get solutions with anti-IgG, anti-IgA, anti-IgM, anti-kappa light chain and anti-lambda light chain ...