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Whole genome sequencing (WGS) is the process of determining the entirety, or nearly the entirety, of the DNA sequence of an organism's genome at a single time. [2] This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast .
In terms of genomic coverage and accuracy, whole genome sequencing can broadly be classified into either of the following: [13] A draft sequence, covering approximately 90% of the genome at approximately 99.9% accuracy; A finished sequence, covering more than 95% of the genome at approximately 99.99% accuracy
The addition of whole genome sequencing (WGS) allowed for identification of strains of TB which could then be put in a chronological cluster map. These cluster maps show the origin of cases and the time in which those cases arose. This gives a much clearer picture of transmission dynamics and allows for better control and prevention of ...
Whole genome sequencing, a laboratory process that determines the complete DNA sequence of an organism's genome at a single time; World Geodetic System, a reference frame for the earth for use in geodesy and navigation; Wideband Global SATCOM, a United States and Australian military communication satellite system
In genetics, imputation is the statistical inference of unobserved genotypes. [1] It is achieved by using known haplotypes in a population, for instance from the HapMap or the 1000 Genomes Project in humans, thereby allowing to test for association between a trait of interest (e.g. a disease) and experimentally untyped genetic variants, but whose genotypes have been statistically inferred ...
The whole genome sequencing technique was first applied to the DNA methylation mapping at single nucleotide resolution to Arabidopsis thaliana in 2008, and shortly after in 2009, the first single-base-resolution DNA methylation map of the entire human genome was created using whole genome bisulfite sequencing.
The genomic epidemiological database for global identification of microorganisms or global microbial identifier [1] is a platform for storing whole genome sequencing data of microorganisms, for the identification of relevant genes and for the comparison of genomes to detect and track-and-trace infectious disease outbreaks and emerging pathogens. [2]
The advent of second-generation sequencing technologies has made it possible to obtain sequence information across the entire bacterial genome at relatively modest cost and effort, and MLST can now be assigned from whole-genome sequence information, rather than sequencing each locus separately as was the practice when MLST was first developed. [15]
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