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Whole genome sequencing (WGS) is the process of determining the entirety, or nearly the entirety, of the DNA sequence of an organism's genome at a single time. [2] This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast .
In terms of genomic coverage and accuracy, whole genome sequencing can broadly be classified into either of the following: [13] A draft sequence, covering approximately 90% of the genome at approximately 99.9% accuracy; A finished sequence, covering more than 95% of the genome at approximately 99.99% accuracy
The addition of whole genome sequencing (WGS) allowed for identification of strains of TB which could then be put in a chronological cluster map. These cluster maps show the origin of cases and the time in which those cases arose. This gives a much clearer picture of transmission dynamics and allows for better control and prevention of ...
Whole genome sequencing, a laboratory process that determines the complete DNA sequence of an organism's genome at a single time; World Geodetic System, a reference frame for the earth for use in geodesy and navigation; Wideband Global SATCOM, a United States and Australian military communication satellite system
In genetics, imputation is the statistical inference of unobserved genotypes. [1] It is achieved by using known haplotypes in a population, for instance from the HapMap or the 1000 Genomes Project in humans, thereby allowing to test for association between a trait of interest (e.g. a disease) and experimentally untyped genetic variants, but whose genotypes have been statistically inferred ...
The process of extensive BAC library creation and tiling path selection, however, make hierarchical shotgun sequencing slow and labor-intensive. Now that the technology is available and the reliability of the data demonstrated, [14] the speed and cost efficiency of whole-genome shotgun sequencing has made it the primary method for genome ...
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The NG50 statistic is the same as N50 except that it is 50% of the known or estimated genome size that must be of the NG50 length or longer. This allows for meaningful comparisons between different assemblies. In the typical case that the assembly size is not more than the genome size, the NG50 statistic will not be more than the N50 statistic.
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