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The strip can be placed directly onto a surface of a wet substance or a few drops of the solution can be dropped onto the universal indicator using dropping equipment. If the test solution is of a dark colour, it is preferable to use a paper universal indicator, such as Hydrion paper .
In and of themselves, pH indicators are usually weak acids or weak bases. The general reaction scheme of acidic pH indicators in aqueous solutions can be formulated as: HInd (aq) + H 2 O (l) ⇌ H 3 O + (aq) + Ind − (aq) where, "HInd" is the acidic form and "Ind −" is the conjugate base of the indicator. Vice versa for basic pH indicators ...
A urine test strip or dipstick is a basic ... from yellow to blue between pH 6 and 9. In the range 5 to 9 the strips show colours that change from orange at pH 5 ...
pH can be measured using indicators, which change color depending on the pH of the solution they are in. By comparing the color of a test solution to a standard color chart, the pH can be estimated to the nearest whole number. For more precise measurements, the color can be measured using a colorimeter or spectrophotometer.
Because the test pad reaction is dependent on pH, false positive results can occur if the urine is highly alkaline. [60] [62] Conventional test strips are not sensitive enough to reliably detect microalbuminuria, a condition in which urine albumin levels are slightly elevated, [64] although dipsticks specialized for this measurement exist. [62]
To prepare a solution for use as pH indicator, dissolve 0.10 g in 8.0 cm 3 N/50 (a.k.a. 0.02 Normal) NaOH and dilute with water to 250 cm 3. To prepare a solution for use as indicator in volumetric work, dissolve 0.1 g in 100 cm 3 of 50% (v/v) ethanol .
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