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In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Without oil, light waves reflect off the slide specimen ...
In light microscopy, oil immersion is a technique used to increase the resolution of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
Becke line test. The Becke line test is a technique in optical mineralogy that helps determine the relative refractive index of two materials. It is done by lowering the stage (increasing the focal distance) of the petrographic microscope and observing which direction the light appears to move. This movement will always go into the material of ...
A condenser (right) and its respective diaphragm (left) A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes.
The light path of a bright-field microscope is extremely simple, no additional components are required beyond the normal light-microscope setup. The light path therefore consists of: a transillumination light source, commonly a halogen lamp in the microscope stand; a condenser lens, which focuses light from the light source onto the sample; an ...
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century. One of the first time-lapse microcinematographic films of cells ever ...
Oil immersion objectives typically have even larger numerical apertures so improved optical sectioning. The resolution in the depth direction (the "z resolution") of a standard wide field microscope depends on the numerical aperture and the wavelength of the light and can be approximated as:
Thin sections under a microscope. A petrographic microscope is a type of optical microscope used to identify rocks and minerals in thin sections. The microscope is used in optical mineralogy and petrography, a branch of petrology which focuses on detailed descriptions of rocks. The method includes aspects of polarized light microscopy (PLM).
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