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Other names in common use include thymidine diphosphoglucose oxidoreductase, TDP-glucose oxidoreductase, RmlB, DESIV, and dTDP-glucose 4,6-hydro-lyase. This enzyme participates in 4 metabolic pathways : nucleotide sugars metabolism , streptomycin biosynthesis , polyketide sugar unit biosynthesis , and biosynthesis of vancomycin group antibiotics .
This is typically seen as a broadening of the alpha-2 zone. Alpha-2 macroglobulin may be elevated in children and the elderly. This is seen as a sharp front to the alpha-2 band. AMG is markedly raised (10-fold increase or greater) in association with glomerular protein loss, as in nephrotic syndrome. Due to its large size, AMG cannot pass ...
Anion exchange protein 2 (AE2) is a membrane transport protein that in humans is encoded by the SLC4A2 gene. [5] [6] AE2 is functionally similar to the Band 3 Cl − /HCO3 − exchange protein. Mice have been used to explore the function of AE2. AE2 contributes to basolateral membrane HCO 3 − transport in the gastrointestinal tract. [7]
The protein substance from the initial abstract is inspected with a 2-D Quant Kit. The initial abstract is diluted to 50 ng/mL combined with 0.1% SDS, 0.1% 2-ME, 0.1 M PBS (pH 7.4), 0.1% BSA, and 0.1% Tween 20, and it is deposited for ELISA at 4 °C playing as the calibration standard solution. [8]
Serum total protein, also known as total protein, is a clinical chemistry parameter representing the concentration of protein in serum. [1] Serum contains many proteins including serum albumin, a variety of globulins, and many others. While it is possible to analyze these proteins individually, total protein is a relatively quick and ...
Immunoprecipitation of intact protein complexes (i.e. antigen along with any proteins or ligands that are bound to it) is known as co-immunoprecipitation (Co-IP). Co-IP works by selecting an antibody that targets a known protein that is believed to be a member of a larger complex of proteins.
[4] [15] This implies that there must be two epitopes on the protein (one to capture the protein and one to detect the protein) for which specific antibodies are available. [15] Other forward phase protein microarrays directly label the samples, however there is often variability in the labeling efficiency for different protein, and often the ...
Perilipin 4, also known as S3-12, is a protein that in humans is encoded by the PLIN4 gene on chromosome 19. [5] [6] It is highly expressed in white adipose tissue, with lower expression in heart, skeletal muscle, and brown adipose tissue. [7] PLIN4 coats lipid droplets in adipocytes to protect them from lipases.