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English: Skeleton of a dog: A – Cervical or Neck Bones (7 in number). B – Dorsal or Thoracic Bones (13 in number, each bearing a rib). C – Lumbar Bones (7 in number).D – Sacral Bones (3 in number). E – Caudal or Tail Bones (20 to 23 in number). 1 – Cranium, or Skull. 2 – Maxilla. 3 – Mandible, or Lower jaw . 4 – Atlas. 5 – Axis.
Cytology is the name given to the branch of biology that deals with the formation, structure and functionality of the cells. [1] Liver cytology specializes in the study of liver cells. The main liver cells are called hepatocytes; however, there are other cells that can be observed in a liver sample such as Kupffer cells (macrophages). [2]
For these purposes, hepatocytes are usually isolated from animal or human [8] whole liver or liver tissue by collagenase digestion, which is a two-step process. In the first step, the liver is placed in an isotonic solution, in which calcium is removed to disrupt cell-cell tight junctions by the use of a calcium chelating agent .
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The function and role of quiescent hepatic stellate cells is unclear. Recent evidence suggests a role as a liver-resident antigen-presenting cell, presenting lipid antigens to and stimulating proliferation of NKT cells. [7] When the liver is damaged, stellate cells can change into an activated state.
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