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  2. Shotgun sequencing - Wikipedia

    en.wikipedia.org/wiki/Shotgun_sequencing

    Due to this size limit, longer sequences are subdivided into smaller fragments that can be sequenced separately, and these sequences are assembled to give the overall sequence. In shotgun sequencing, [ 1 ] [ 2 ] DNA is broken up randomly into numerous small segments, which are sequenced using the chain termination method to obtain reads .

  3. Glossary of biology - Wikipedia

    en.wikipedia.org/wiki/Glossary_of_biology

    This glossary of biology terms is a list of definitions of fundamental terms and concepts used in biology, the study of life and of living organisms.It is intended as introductory material for novices; for more specific and technical definitions from sub-disciplines and related fields, see Glossary of cell biology, Glossary of genetics, Glossary of evolutionary biology, Glossary of ecology ...

  4. Glossary of cellular and molecular biology (M–Z) - Wikipedia

    en.wikipedia.org/wiki/Glossary_of_cellular_and...

    Also nuclear localization sequence. An amino acid sequence within a protein which serves as a molecular signal marking the protein for transport into the nucleus, typically consisting of one or more short motifs containing positively charged amino acid residues exposed on the mature protein's surface (especially lysines and arginines).

  5. Glossary of genetics and evolutionary biology - Wikipedia

    en.wikipedia.org/wiki/Glossary_of_genetics_and...

    Also called functionalism. The Darwinian view that many or most physiological and behavioral traits of organisms are adaptations that have evolved for specific functions or for specific reasons (as opposed to being byproducts of the evolution of other traits, consequences of biological constraints, or the result of random variation). adaptive radiation The simultaneous or near-simultaneous ...

  6. RNA-Seq - Wikipedia

    en.wikipedia.org/wiki/RNA-Seq

    After sequencing, read counts of spike-in sequences are used to determine the relationship between each gene's read counts and absolute quantities of biological fragments. [ 13 ] [ 98 ] In one example, this technique was used in Xenopus tropicalis embryos to determine transcription kinetics.

  7. Sequence analysis - Wikipedia

    en.wikipedia.org/wiki/Sequence_analysis

    However, comparing these new sequences to those with known functions is a key way of understanding the biology of an organism from which the new sequence comes. Thus, sequence analysis can be used to assign function to coding and non-coding regions in a biological sequence usually by comparing sequences and studying similarities and differences.

  8. Amplicon sequence variant - Wikipedia

    en.wikipedia.org/wiki/Amplicon_sequence_variant

    An amplicon sequence variant (ASV) is any one of the inferred single DNA sequences recovered from a high-throughput analysis of marker genes. Because these analyses, also called "amplicon reads," are created following the removal of erroneous sequences generated during PCR and sequencing, using ASVs makes it possible to distinguish sequence ...

  9. Glossary of cellular and molecular biology (0–L) - Wikipedia

    en.wikipedia.org/wiki/Glossary_of_cellular_and...

    3' untranslated region (3'-UTR). Also three-prime untranslated region, 3' non-translated region (3'-NTR), and trailer sequence.. 3'-end. Also three-prime end.. One of two ends of a single linear strand of DNA or RNA, specifically the end at which the chain of nucleotides terminates at the third carbon atom in the furanose ring of deoxyribose or ribose (i.e. the terminus at which the 3' carbon ...