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Chromatographic peak resolution is given by = + where t R is the retention time and w b is the peak width at baseline. The bigger the time-difference and/or the smaller the bandwidths, the better the resolution of the compounds. Here compound 1 elutes before compound 2.
This can be done by choosing a stationary phase that has a greater difference between k 1 ' and k 2 '. It can also be done in L.C. by using pH to invoke secondary equilibria (if applicable). The fundamental resolution equation is derived as follows: For two closely spaced peaks, ω 1 = ω 2, and σ 1 = σ 2. so R s = (t r2 - t r1)/ω 2 = (t r2 ...
Resolution and resolving power, when defined in this way, are consistent with IUPAC recommendations for microscopy, optical spectroscopy. [16] [17] and ion microscopy (SIMS) [18] but not gas chromatography. [13] This definition also appears in some mass spectrometry texts. [19] [20] [21]
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The response factor can be expressed on a molar, volume or mass [1] basis. Where the true amount of sample and standard are equal: = where A is the signal (e.g. peak area) and the subscript i indicates the sample and the subscript st indicates the standard. [2]
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