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Bile Esculin Agar (BEA) is a selective differential agar used to isolate and identify members of the genus Enterococcus, [1] formerly part of the "group D streptococci" (enterococci were reclassified in their own genus in 1984).
Most strains are gamma-hemolytic (non-hemolytic), but some also display alpha-hemolytic activity on sheep blood agar plates. Strep bovis is a non-enterococci. [citation needed] Biochemical Tests mannitol salt: negative bile esculin: negative MR/VP: positive/negative nutrient gelatin: negative starch: positive DNase: negative [citation needed]
Aesculin is incorporated into agar with ferric citrate and bile salts (bile aesculin agar). [5] Hydrolysis of aesculin forms aesculetin (6,7-dihydroxycoumarin) and glucose. Aesculetin forms dark brown or black complexes with ferric citrate, allowing the test to be read. The bile aesculin agar is streaked and incubated at 37 °C (99 °F) for 24 ...
MacConkey agar is a selective and differential medium used to differentiate between gram-negative bacteria while inhibiting the growth of gram-positive bacteria. Adding bile salts and crystal violet to the agar inhibits the growth of most gram-positive bacteria, making MacConkey agar selective.
Aerobic and anaerobic bacteria can be identified by growing them in test tubes of thioglycolate broth: 1: Obligate aerobes need oxygen because they cannot ferment or respire anaerobically.
E. coli on EMB agar. Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective and differential media used for the identification of Gram-negative bacteria, [1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample.
The bacterium is catalase-negative and LAP-positive (like all streptococci), PYR-test and CAMP-test-positive, does not hydrolyze sodium hippurate, and does not grow in bile esculin agar. [21] It does not express any of the known Lancefield antigens .
Bilophila wadsworthia is a Gram-negative, obligately anaerobic, catalase-positive, bile-resistant, and asaccharolytic bacillus. Approximately 75% of B. wadsworthia strains are urease positive. B. wadsworthia is linked to various diseases and is not well known due to frequent misidentification of the bacteria, and the National Center for ...