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Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the specimens (for positive stains) or background (for negative stains) will be one color. Therefore, simple stains are typically used for viewing only one organism per slide. Differential staining uses multiple stains per ...
Histologic specimen being placed on the stage of an optical microscope Human lung tissue stained with hematoxylin and eosin as seen under a microscope. Histology, [help 1] also known as microscopic anatomy or microanatomy, [1] is the branch of biology that studies the microscopic anatomy of biological tissues.
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
Anatomical terminology is used to describe microanatomical (or histological) structures. This helps describe precisely the structure, layout and position of an object, and minimises ambiguity. An internationally accepted lexicon is Terminologia Histologica.
The Wayson stain is a basic fuchsin-methylene blue, ethyl alcohol-phenol microscopic staining procedure. It was originally a modified methylene blue stain used for diagnosing bubonic plague . [ 1 ] With this stain, Yersinia pestis appears purple with a characteristic safety-pin appearance, [ 2 ] which is due to the presence of a central vacuole.
Movat's stain is a pentachrome stain originally developed by Henry Zoltan Movat (1923–1995), a Hungarian-Canadian Pathologist in Toronto [1] in 1955 to highlight the various constituents of connective tissue, especially cardiovascular tissue, by five colors in a single stained slide. [2]
In pathology, the Grocott–Gömöri's methenamine silver stain, abbreviated GMS, is a popular staining method in histology. The stain was originally named after György Gömöri, the Hungarian physician who developed the stain. It is used widely as a screen for fungal organisms. It is particularly useful in staining carbohydrates.
To perform immunofluorescence staining, a fluorophore must be conjugated (“tagged”) to an antibody. Staining procedures can be applied to both retained intracellular expressed antibodies, or to cell surface antigens on living cells. There are two general classes of immunofluorescence techniques: primary (direct) and secondary (indirect).