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A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
In contrast, basic dyes are used to stain cell nuclei and some other acidic components of tissues. [8] Regarding cellular structures, acid dyes will stain acidophilic structures that have a net positive charge due to the fact that they have a negatively charged chromophore. Acidophilic structures include the cytoplasm, collagen and mitochondria ...
Gram-positive cells have a thick layer of peptidoglycan in the cell wall that retains the primary stain, crystal violet. Gram-negative cells have a thinner peptidoglycan layer that allows the crystal violet to wash out on addition of ethanol. They are stained pink or red by the counterstain, [3] commonly safranin or fuchsine.
A "vital dye" or stain is a dye capable of penetrating living cells or tissues without causing immediate visible degenerative changes. [26] Such dyes are useful in medical and pathological fields in order to selectively color certain structures (such as cells) in order to distinguish them from surrounding tissue and thus make them more visible ...
The Ziehl-Neelsen stain is a two step staining process. In the first step, the tissue is stained with a basic fuchsin solution, which stains all cells pink. In the second step, the tissue is incubated in an acid alcohol solution, which decolorizes all cells except for acid-fast cells, which retain the color and appeared as red.
[9] [19] Hematoxylin stains cell nuclei blue; eosin, an acidic dye, stains the cytoplasm and other tissues in different stains of pink. [ 9 ] [ 12 ] In contrast to H&E, which is used as a general stain, there are many techniques that more selectively stain cells, cellular components, and specific substances. [ 12 ]
It is used as a food coloring, [4] printing ink, [5] biological stain, [6] dental plaque disclosing agent, [7] radiopaque medium, [6] sensitizer for orthochromatic photographic films, and visible light photoredox catalyst. [8] Erythrosine is commonly used in sweets such as some candies and ice pops, and even more widely used in cake-decorating ...
Phloxine B (commonly known simply as phloxine) is a water-soluble red dye used for coloring drugs and cosmetics in the United States [2] and coloring food in Japan. [3] It is derived from fluorescein, but differs by the presence of four bromine atoms at positions 2, 4, 5 and 7 of the xanthene ring and four chlorine atoms in the carboxyphenyl ring. [4]