Ads
related to: phosphatase inhibitor cocktail 2 sigma
Search results
Results from the WOW.Com Content Network
Protease inhibitors (1 mM PMSF (fresh from 1 M stock in i-propanol), 1 μg/mL each leupeptin, aprotinin, pepstatin, 1-5 mM EDTA, 0.5-1 mM EGTA, 5 mM aminocaproic acid) or commercial protease inhibitor cocktail (use according to the manufacturer's instruction) 1 mM each Na 3 VO 4 and Na 4 P 2 O 7 as phosphatase inhibitor (if phosphorylation ...
Protein phosphatase 2 (PP2), also known as PP2A, is an enzyme that in humans is encoded by the PPP2CA gene. [ 2 ] [ 3 ] The PP2A heterotrimeric protein phosphatase is ubiquitously expressed, accounting for a large fraction of phosphatase activity in eukaryotic cells. [ 4 ]
β-Glycerophosphate is an inhibitor of the enzyme serine/threonine phosphatase. It is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. [1] [2] β-Glycerophosphate is also used to drive osteogenic differentiation of bone marrow stem cells in vitro. [3]
The half-life is short in aqueous solutions (110 min at pH 7, 55 min at pH 7.5, and 35 min at pH 8, all at 25 °C). [2] At 4˚C, pH 8, PMSF is almost completely degraded after 1 day. [ 2 ] Stock solutions are usually made up in anhydrous ethanol , isopropanol , or corn oil and diluted immediately before use.
Serine and threonine phosphates are stable under physiological conditions, so a phosphatase enzyme has to remove the phosphate to reverse the regulation signal. Ser/Thr-specific protein phosphatases are regulated partly by their location within the cell and by specific inhibitor proteins.
Pages in category "Phosphatase inhibitors" The following 13 pages are in this category, out of 13 total. ... This page was last edited on 2 April 2018, ...
0.01 M sodium phosphate, pH 7.2; 2 mM EDTA; 50 mM sodium fluoride (NaF) 0.2 mM fresh sodium orthovanadate (Na 3 VO 4.2H 2 O, it has phosphatase inhibitor function because it mimics phosphate) 100 U/ml protease inhibitor, such as aprotinin
PHLPP is a member of the PPM family of phosphatases, which requires magnesium or manganese for their activity and are insensitive to most common phosphatase inhibitors, including [okadaic acid]. PHLPP1 and PHLPP2 have a similar domain structure, which includes a putative Ras association domain, a pleckstrin homology domain , a series of leucine ...
Ads
related to: phosphatase inhibitor cocktail 2 sigma