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The air in the incubator was kept at 37 degrees Celsius, the same temperature as the human body, and the incubator maintained the atmospheric carbon dioxide and nitrogen levels necessary to promote cell growth. At this time, incubators also began to be used in genetic engineering. Scientists could create biologically essential proteins, such as ...
Single or sequential medium are equally effective for the culture of human embryos to the blastocyst stage. [7] Artificial embryo culture media basically contain glucose, pyruvate, and energy-providing components, but the addition of amino acids, nucleotides, vitamins, and cholesterol improve the performance of embryonic growth and development.
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Incubator (culture), a device used to grow and maintain microbiological cultures or cell cultures; Incubator (egg), a device for maintaining the eggs of birds or reptiles to allow them to hatch; Incubator (neonatal), a device used to care for premature babies in a neonatal intensive-care unit
Tissue culture flasks. RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [2]
Stem-cell lines are grown and maintained at specific temperature and atmospheric conditions (37 degrees Celsius and 5% CO 2) in incubators. Culture conditions such as the cell growth medium and surface on which cells are grown vary widely depending on the specific stem cell line. Different biochemical factors can be added to the medium to ...
In addition, control of the fluid dynamics within each hollow fiber bioreactor led to further optimization of the cell culture environment. By alternating the pressure gradient across the hollow fiber membrane, media could flow back and forth between the EC side (cell compartment) and the IC side (hollow fiber lumen). This process, combined ...
A key component of microfluidic cell culture is being able to mimic the cell microenvironment which includes soluble factors that regulate cell structure, function, behavior, and growth. [2] Another important component for the devices is the ability to produce stable gradients that are present in vivo as these gradients play a significant role ...
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