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One example is capillary electrophoresis, [10] [12] in which electric fields are used to separate chemicals according to their electrophoretic mobility by applying an electric field to a narrow capillary, usually made of silica. In electrophoretic separations, the electroosmotic flow affects the elution time of the analytes.
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
[1] [2] Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube , which is filled with the analyte.
In this method a high voltage is applied to the sample solution and molecules are loaded to the CE capillary by electromigration and electroosmotic flow of the sample. [10] Electrokinetic injection improves the sensitivity comparing to hydrodynamic injection while using lower voltage and longer injection time, but reproducibility of peak areas ...
Capillary electrochromatography (CEC) is an electrochromatography technique in which the liquid mobile phase is driven through a capillary containing the chromatographic stationary phase by electroosmosis. [3] [4] It is a combination of high-performance liquid chromatography and capillary electrophoresis. The capillaries is packed with HPLC ...
The surface potential of the cell wall produces electro-osmotic flow. Since the electrophoresis chamber is a closed system, backward flow is produced at the center of the cell. Then the observed mobility or velocity from Eq. (7) is a result of the combination of osmotic flow and electrophoretic movement.
The EE method was first applied to analytical chemistry by Van der Vlis in 1994. [7] A diagram of an electroextraction apparatus is shown in figure 2. The apparatus consists of a vial with a conical bottom, a grounded platinum electrode, a capillary to inject the aqueous solution, and an adjustable gold anode with a circular bottom that ...
Flow-induced dispersion analysis (FIDA) is an immobilization-free technology used for characterization and quantification of biomolecular interaction and protein concentration under native conditions. [1] [2] [3] In the FIDA assay, the size of a ligand (indicator) with affinity to the target analyte is measured. When the indicator interacts ...