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Reservoir plates are also commercially available. [24] Reservoir plates have columns of wells (as in 96-well, 24-well, etc. plates) that are fused into single wells, so that they provide additional volume for multichannel pipettes. Like deepwell plates or blocks, they often follow a de facto standard height of 44 mm.
Sample reactions can be assayed in 1-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 μL per well.
Fractionation at total reflux. The Fenske equation in continuous fractional distillation is an equation used for calculating the minimum number of theoretical plates required for the separation of a binary feed stream by a fractionation column that is being operated at total reflux (i.e., which means that no overhead product distillate is being withdrawn from the column).
For manual high-throughput applications like filling up a 96-well microtiter plate most researchers prefer a multi-channel pipette. Instead of handling well by well, a row of 8 wells can be handled in parallel as this type of pipette has 8 pistons in parallel. Adjustable tip spacing pipette transferring samples from a 384 well plate to a 96 ...
This assay is presumably very easy to do on a 24-well plate and read with a plate reader; i wouldn't want to count twenty-odd samples on a haemocytometer. The formation of the dye is catalysed by mitochondrial enzymes, so this assay really measures the quantity of those enzymes, right?
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The plates are left upright on the bench to dry before inversion and incubation at 37 °C for 18 – 24 hours (or appropriate incubation conditions considering the organism and agar used). Each sector is observed for growth, high concentrations will give a confluent growth over the area of the drop, or a large number of small/merged colonies.
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