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Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. [2] While the first cases of IHC staining used fluorescent dyes (see immunofluorescence ), other non-fluorescent methods using enzymes such as peroxidase (see ...
Drawing by Camillo Golgi of a hippocampus stained with the silver nitrate method Drawing of a Purkinje cell in the cerebellum cortex done by Santiago Ramón y Cajal, clearly demonstrating the power of Golgi's staining method to reveal fine detail. Golgi's method is a silver staining technique that is used to visualize nervous tissue under light ...
Ribbon diagram of myoglobin bound to haem (sticks) and oxygen (red spheres) (Ribbon diagrams, also known as Richardson diagrams, are 3D schematic representations of protein structure and are one of the most common methods of protein depiction used today. The ribbon depicts the general course and organization of the protein backbone in 3D and ...
The left figure below shows a binary decision tree (the reduction rules are not applied), and a truth table, each representing the function (,,).In the tree on the left, the value of the function can be determined for a given variable assignment by following a path down the graph to a terminal.
The optics do not change the color of the specimen, making it easy to interpret what is observed. Bright-field microscopy is a standard light-microscopy technique, and therefore magnification is limited by the resolving power possible with the wavelength of visible light .
The purpose of this technique is to analyze the activity of a gene transcription promoter (in terms of expression of a so-called reporter gene under the regulatory control of that promoter) either in a quantitative manner, involving some measure of activity, or qualitatively (on versus off) through visualization of its activity in different cells, tissues, or organs.
DAPI can be used for fixed cell staining. The concentration of DAPI needed for live cell staining is generally very high; it is rarely used for live cells. [ 7 ] It is labeled non-toxic in its MSDS [ 8 ] and though it was not shown to have mutagenicity to E. coli , [ 9 ] it is labelled as a known mutagen in manufacturer information. [ 2 ]
Picture of an SDS-PAGE. The molecular markers (ladder) are in the left lane. Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.