Search results
Results from the WOW.Com Content Network
The role of arginine as an enhancer has been investigated in Dendroides canadensis antifreeze protein (DAFP-1) by observing the effect of a chemical modification using 1-2 cyclohexanedione. [39] Previous research has found various enhancers of this bettles' antifreeze protein including a thaumatin-like protein and polycarboxylates.
A cryoprotectant is a substance used to protect biological tissue from freezing damage (i.e. that due to ice formation). Arctic and Antarctic insects, fish and amphibians create cryoprotectants (antifreeze compounds and antifreeze proteins) in their bodies to minimize freezing damage during cold winter periods.
Antifreeze proteins bind to small ice crystals to inhibit growth and recrystallization of ice that would otherwise be fatal. [ 5 ] [ 6 ] Cryoprotectants are commonly used in cryobiology to prevent or inhibit freezing in sperm, blood, stem cells, plant seeds, etc. [ 7 ] [ 8 ] Ethylene glycol, propylene glycol, and glycerol (all used in ...
Whereas most insect antifreeze proteins contain cysteines at least every sixth residue, as well as varying numbers of 12- or 13-mer repeats of 8.3-12.5kDa, [4] RiAFP is notable for containing only one disulfide bridge. This property of RiAFP makes it particularly attractive for recombinant expression and biotechnological applications.
Certain proteins, named ice restructuring proteins or antifreeze proteins, stop small ice crystals from recrystallizing into larger crystals that can cause physical damage to tissues. Cryoprotectants: These are several factors that prevent intracellular freezing, prevent excessive reduction of cell volume, and stabilize protein conformation.
Proteins also play a large role in cold hardening. Glycogen phosphorylase (GlyP) is a key enzyme that increases in comparison to a control group not experiencing cold hardening. [ 8 ] Once warmer temperatures are observed, the process of acclimation begins, and the increase in the concentrations of glycerol and other cryoprotective compounds is ...
A seasonal photoperiodic timing mechanism is responsible for increasing the antifreeze protein levels with concentrations reaching their highest in the winter. In the pyrochroid beetle, Dendroides canadensis , a short photoperiod of 8 hours light and 16 hours of darkness, results in the highest levels of THFs, [ 21 ] which corresponds with the ...
In June 2006, the Unilever company announced that it would research the potential use of genetically modified yeast to grow antifreeze proteins based on a gene from the ocean pout, and use proteins extracted from the yeast to improve the consistency and storage properties of ice cream. Incorporating these ice-structuring proteins means that a ...