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Chromatographic peak resolution is given by = + where t R is the retention time and w b is the peak width at baseline. The bigger the time-difference and/or the smaller the bandwidths, the better the resolution of the compounds.
In liquid chromatography, the mobile phase velocity is taken as the exit velocity, that is, the ratio of the flow rate in ml/second to the cross-sectional area of the ‘column-exit flow path.’ For a packed column, the cross-sectional area of the column exit flow path is usually taken as 0.6 times the cross-sectional area of the column.
A high value for resolution corresponding to good separation of peaks is similar to the convention used with chromatography separations, [13] although it is important to note that the definitions are not the same. [14] High resolution indicating better peak separation is also used in ion mobility spectrometry. [15]
Chromatography software is called also Chromatography Data System. [1] It is located in the data station of the modern liquid, gas or supercritical fluid chromatographic systems. This is a dedicated software connected to an hardware interface within the chromatographic system, which serves as a central hub for collecting, analyzing, and ...
A mass chromatogram is a representation of mass spectrometry data as a chromatogram, where the x-axis represents time and the y-axis represents signal intensity. [1] The source data contains mass information; however, it is not graphically represented in a mass chromatogram in favor of visualizing signal intensity versus time.
Closely related compounds have similar chemical properties that may prove difficult to separate based on polarity, charge, etc. [15] Two-dimensional liquid chromatography provides separation based on more than one chemical or physical property. Using an example from Nagy and Vekey, a mixture of peptides can be separated based on their basicity ...
The charged aerosol detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer.
A relatively recent analytical tool that has been used for the separation of UCMs is comprehensive two-dimensional GC ().This powerful technique, introduced by Liu and Phillips [27] combines two GC columns with different separation mechanisms: typically a primary column that separates compounds based on volatility coupled to a second short column that separates by polarity.