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Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
In both cases the numerical aperture of the objective is 1.49 and the refractive index of the medium 1.52. The wavelength of the emitted light is assumed to be 600 nm and, in case of the confocal microscope, that of the excitation light 500 nm with circular polarization. A section is cut to visualize the internal intensity distribution.
Raman microscopy, and in particular confocal microscopy, can reach down to sub-micrometer lateral spatial resolution. [7] Because a Raman microscope is a diffraction-limited system, its spatial resolution depends on the wavelength of light and the numerical aperture of the focusing element. In confocal Raman microscopy, the diameter of the ...
BS 546, Two-pole and earthing-pin plugs, socket-outlets and socket-outlet adaptors for AC (50–60 Hz) circuits up to 250 V is an older British Standard for three-pin AC power plugs and sockets: four sizes with current capacities from 2 A to 30 A. Originally published in April 1934, it was updated by a 1950 edition which is still current, [1 ...
The GU24 fitting is intended to maintain the energy efficiency of the light by preventing an occupant from using an incandescent bulb instead of a CFL. Adapters to use incandescent bulbs in a GU24 fitting are illegal in the State of California as they would be a fire hazard in fixtures designed for the lower heat output of a CFL bulb.
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.
Scanning confocal electron microscopy (SCEM) is an electron microscopy technique analogous to scanning confocal optical microscopy (SCOM). In this technique, the studied sample is illuminated by a focussed electron beam, as in other scanning microscopy techniques, such as scanning transmission electron microscopy or scanning electron microscopy .
A lightbulb socket, lightbulb holder, light socket, lamp socket or lamp holder is a device which mechanically supports and provides electrical connections for a compatible electric lamp base. [1] Sockets allow lamps to be safely and conveniently replaced (re-lamping).
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