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A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl 2 •2H 2 O), with 9.95 mL of 1% sulfuric acid (H 2 SO 4). [1] Now there are McFarland standards prepared from suspensions of latex particles, which lengthens the shelf life and stability of the suspensions. The standard can be compared visually to ...
The first alkaline lysis was performed by Birnom and Doly in 1979. [1] Since then, slight modifications have been made to the procedure to get to today's most widely used approach. The steps of alkaline lysis can be summarized as the formation of a pellet, resuspension of the pellet in solution, cell lysis, neutralization, and centrifugation.
A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
NetPrimer is a gratis web-based tool used for analysing primers used in PCR to amplify a DNA sequence. [2] The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR ...
In summary, in order to find a standard curve, one must use varying concentrations of BSA (Bovine Serum Albumin) [2] in order to create a standard curve with concentration plotted on the x-axis and absorbance plotted on the y-axis. Only a narrow concentration of BSA is used (2-10 ug/mL) in order to create an accurate standard curve. [23]
Factors such as buffer, charge/voltage, and concentration of gel can affect the mobility and/or appearance of your marker/ladder/standard. These elements need to be taken into consideration when selecting a marker and when analyzing the final results on a gel. 1.2% agarose gel showing two different DNA ladders dyed with GelRed stain Buffers
A primary standard in metrology is a standard that is sufficiently accurate such that it is not calibrated by or subordinate to other standards. Primary standards are defined via other quantities like length, mass and time. Primary standards are used to calibrate other standards referred to as working standards. [1] [2] See Hierarchy of Standards.
IC 50 is a quantitative measure that indicates how much of a particular inhibitory substance (e.g. drug) is needed to inhibit, in vitro, a given biological process or biological component by 50%. [1] The biological component could be an enzyme, cell, cell receptor or microbe. IC 50 values are typically expressed as molar concentration.