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Viral transformation is the change in growth, phenotype, or indefinite reproduction of cells caused by the introduction of inheritable material. Through this process, a virus causes harmful transformations of an in vivo cell or cell culture.
Viral replication is the formation of biological viruses during the infection process in the target host cells. Viruses must first get into the cell before viral replication can occur. Through the generation of abundant copies of its genome and packaging these copies, the virus continues infecting new hosts. Replication between viruses is ...
An example is the viral transfer of DNA from one bacterium to another and hence an example of horizontal gene transfer. [2] [3] [page needed] Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA (which occurs in conjugation), and it is DNase resistant (transformation is
Viral vector based gene delivery uses a viral vector to deliver genetic material to the host cell. This is done by using a virus that contains the desired gene and removing the part of the viruses genome that is infectious. [2] Viruses are efficient at delivering genetic material to the host cell's nucleus, which is vital for replication. [2]
Cytopathic effect (abbreviated CPE) refers to structural changes in host cells that are caused by viral invasion. The infecting virus causes lysis of the host cell or when the cell dies without lysis due to an inability to replicate. [1] If a virus causes these morphological changes in the host cell, it is said to be cytopathogenic. [2]
To enter the cells, proteins on the surface of the virus interact with proteins of the cell. Attachment, or adsorption, occurs between the viral particle and the host cell membrane. A hole forms in the cell membrane, then the virus particle or its genetic contents are released into the host cell, where replication of the viral genome may commence.
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Viral vectors are employed for cellular reprogramming, like inducing pluripotent stem cells or differentiating adult somatic cells into different cell types. [9] Researchers also use viral vectors to create transgenic mice and rats for experiments. [10] Viral vectors can be used for in vivo imaging via the introduction of a reporter gene.