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Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
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Pyrosequencing, a new method of DNA sequencing which involves the addition of phosphorylated dNTP's to the DNA polymerase reaction which emmit light upon binding to the DNA template. It makes DNA sequencing significantly faster than with the current chain termination method. We do use them, look at Pyrosequencing/Temp.
Right-click on the graph and select "Export" → "Encapsulated Postscript" from the menu which appears. Choose a file name to save the graph as. In Inkscape, import the graph using "File" → "Import...". After importing, select "File" → "Document Properties..." and click "Fit page to selection". Save the SVG file and upload it.
Another limitation of this system is the short read length compared to other sequencing methods such as Sanger sequencing or pyrosequencing. Longer read lengths are beneficial for de novo genome assembly. Ion Torrent semiconductor sequencers produce an average read length of approximately 400 nucleotides per read. [3] [8]