Search results
Results from the WOW.Com Content Network
Plate count agar (PCA), also called standard methods agar (SMA), is a microbiological growth medium commonly used to assess or to monitor "total" or viable bacterial growth of a sample. PCA is not a selective medium.
The laboratory procedure involves making serial dilutions of the sample (1:10, 1:100, 1:1000, etc.) in sterile water and cultivating these on nutrient agar in a dish that is sealed and incubated. Typical media include plate count agar for a general count or MacConkey agar to count Gram-negative bacteria such as E. coli. Typically one set of ...
Plate count agar in microbiology; Polymerase cycling assembly, for large DNA oligonucleotides; Posterior cerebral artery; Posterior cortical atrophy, a form of dementia; Prostate cancer; Protein-fragment complementation assay, to identify protein–protein interactions; Protocatechuic acid, a phenolic acid.
The phage can then be isolated from the resulting plaques in a lawn of bacteria on a plate. Viral cultures are obtained from their appropriate eukaryotic host cells. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar ...
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
The discovery process included isolation of the research samples, cultivation, microscopic examination, and various biochemical tests. Initially, S. simulans strains were identified in dry sausage samples by plating them on a selective medium for Staphylococcus, as designed by Schleifer and Krämer, or alternatively on plate-count agar. [1]
Colony-forming units are used to quantify results in many microbiological plating and counting methods, including: The pour plate method wherein the sample is suspended in a Petri dish using molten agar cooled to approximately 40–45 °C (just above the point of solidification to minimize heat-induced cell death).
Eaton's agar is a type of agar media is used to grow Mycoplasma pneumoniae. One recipe for the cultivation of M. pneumoniae (Eaton's agar) includes : [1] 70% Difco PPLO (pleuropneumonia-like organism) agar or broth base; 20% unheated horse serum; 10% fresh aqueous extract of baker's yeast; 1000 units/ml Penicillin G