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Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, [4] whereas CISH probes are labelled with biotin or digoxigenin [5] and can be detected using a bright-field microscope after other treatment steps have ...
A traditional exam involving metaphase chromosome analysis is often unable to identify features that distinguish one disease from another, due to subtle chromosomal features; FISH can elucidate these differences. FISH can also be used to detect diseased cells more easily than standard Cytogenetic methods, which require dividing cells and ...
Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...
Between 333 and 322 BC, he provided the earliest taxonomic classification of fish, accurately describing 117 species of Mediterranean fish. [4] Furthermore, Aristotle documented anatomical and behavioral differences between fish and marine mammals. After his death, some of his pupils continued his ichthyological research.
An effective way to clear Ich from a fish population is to transfer all of the fish carrying trophonts in their skin, fins or gills to a non-infected tank every 24 hours. Then the fish do not get re-infected and after a number of days (dependent on temperature) the fish have cleared the infection because trophonts exit within this period.
A number of medications and water treatments are used in this way. Salt is the most effective bath treatment, and is used to eliminate ciliated protozoan parasites (including ich in small fish); also used to curb the absorption of nitrite, and to reduce the osmotic pressure exerted by fresh-water on any hole in the skin or gill.
Fishes are a paraphyletic group and for this reason, the class Pisces seen in older reference works is no longer used in formal taxonomy.Traditional classification divides fish into three extant classes (Agnatha, Chondrichthyes, and Osteichthyes), and with extinct forms sometimes classified within those groups, sometimes as their own classes: [1]
Pollachius virens is a species of marine fish in the genus Pollachius.Together with P. pollachius, it is generally referred to in the United States as pollock.It is commonly known in Britain as the coalfish, coley, or saithe (/ s eɪ ð / or / s eɪ θ /), [1] [2] and the young fish may also be called podleys in Scotland and northern England.