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Rolling circle replication produces multiple copies of a single circular template. Rolling circle replication ( RCR ) is a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA or RNA , such as plasmids , the genomes of bacteriophages , and the circular RNA genome of viroids .
Workflow for DNA nanoball sequencing [1] DNA nanoball sequencing is a high throughput sequencing technology that is used to determine the entire genomic sequence of an organism. The method uses rolling circle replication to amplify small fragments of genomic DNA into DNA nanoballs. Fluorescent nucleotides bind to complementary nucleotides and ...
In the single stranded DNA viruses—a group that includes the circoviruses, the geminiviruses, the parvoviruses and others—and also the many phages and plasmids that use the rolling circle replication (RCR) mechanism, the RCR endonuclease creates a nick in the genome strand (single stranded viruses) or one of the DNA strands (plasmids).
After Minecraft was fully released in November 2011, the game's modding community continued to grow. [16] In February 2012, Mojang hired developers of Bukkit to work on an official modding API, allowing mod developers easier access to the Minecraft game files. [21] Bukkit was then maintained by the community.
Each D-loop contains an origin of replication for the heavy strand. Full circular DNA replication is initiated at that origin and replicates in only one direction. The middle strand in the D-loop can be removed and a new one will be synthesized that is not terminated until the heavy strand is fully replicated, or the middle strand can serve as a primer for the heavy strand replication.
Rolling-Circle Mechanism for Helitron transposition and gene acquisition in the concerted model Helitron could be either autonomous or non-autonomous. One transposase molecule cleaves at the donor (by the first tyrosine (Y1) residue of the Rep protein) and target sites (by the second tyrosine (Y2) residue) and binds to the resulting 5' ends.
Parvoviruses replicate their genomes through a process called rolling hairpin replication (RHR), which is a unidirectional, strand displacement form of DNA replication. Before replication, the coding portion of the ssDNA genome is converted to a double-strand DNA (dsDNA) form, which is then cleaved by a viral protein to initiate replication.
PcrA, standing for plasmid copy reduced is a helicase that was originally discovered in a screen for chromosomally encoded genes that are affected in plasmid rolling circle replication in the Gram-positive pathogen Staphylococcus aureus.
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