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Gene length: Longer genes will have more fragments/reads/counts than shorter genes if transcript expression is the same. This is adjusted by dividing the FPM by the length of a feature (which can be a gene, transcript, or exon), resulting in the metric fragments per kilobase of feature per million mapped reads (FPKM). [90]
Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
One quadrillionth of a second. Pulse time on fastest lasers. svedberg: 10 −13 s: Time unit used for sedimentation rates (usually of proteins). picosecond: 10 −12 s: One trillionth of a second. nanosecond: 10 −9 s: One billionth of a second. Time for molecules to fluoresce. shake: 10 −8 s: 10 nanoseconds, also a casual term for a short ...
While single-read accuracy is 87%, consensus accuracy has been demonstrated at 99.999% with multi-kilobase read lengths. [ 37 ] [ 38 ] In 2015, Pacific Biosciences released a new sequencing instrument called the Sequel System, which increases capacity approximately 6.5-fold.
There are two common methods in which to construct a DNA molecular-weight size marker. [3] One such method employs the technique of partial ligation. [3] DNA ligation is the process by which linear DNA pieces are connected to each other via covalent bonds; more specifically, these bonds are phosphodiester bonds. [4]
One billionth of one second 1 ns: The time needed to execute one machine cycle by a 1 GHz microprocessor 1 ns: The time light takes to travel 30 cm (11.811 in) 10 −6: microsecond: μs One millionth of one second 1 μs: The time needed to execute one machine cycle by an Intel 80186 microprocessor 2.2 μs: The lifetime of a muon
Measuring user engagement in seconds may make it tougher for bots to game the platform, but it won't bring real users back. Elon Musk’s new ‘user seconds’ metric is another curious chapter ...
"Most agarose gels are made with between 0.7% (good separation or resolution of large 5–10kb DNA fragments) and 2% (good resolution for small 0.2–1kb fragments) agarose dissolved in electrophoresis buffer. Up to 3% can be used for separating very tiny fragments but a vertical polyacrylamide gel is more appropriate in this case.