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Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides. [1] An example Maxam–Gilbert sequencing reaction.
Methods and Concepts in the Life Sciences/DNA Sequencing; Usage on es.wikipedia.org Secuenciación Maxam-Gilbert; Usage on fa.wikipedia.org توالییابی به روش مکسام-گیلبرت; Usage on gl.wikipedia.org Secuenciación de Maxam e Gilbert; Usage on he.wikipedia.org ריצוף מקסאם-גילברט; Usage on ko.wikipedia.org
Allan Maxam (born October 28, 1942) is one of the pioneers of molecular genetics. He was one of the contributors to develop a DNA sequencing method at Harvard University , while working as a student in the laboratory of Walter Gilbert .
The technique known as the “Plus and Minus” method, involved supplying all the components of the DNA but excluding the reaction of one of the four bases needed to complete the DNA. [44] In 1976, Gilbert and Maxam, invented a method for rapidly sequencing DNA while at Harvard, known as the Maxam–Gilbert sequencing. [45]
Automated DNA-sequencing instruments (DNA sequencers) can sequence up to 384 DNA samples in a single batch. Batch runs may occur up to 24 times a day. Batch runs may occur up to 24 times a day. DNA sequencers separate strands by size (or length) using capillary electrophoresis , they detect and record dye fluorescence, and output data as ...
The method uses an enzyme, deoxyribonuclease (DNase, for short), to cut the radioactively end-labeled DNA, followed by gel electrophoresis to detect the resulting cleavage pattern. For example, the DNA fragment of interest may be PCR amplified using a 32 P 5' labeled primer, with the result being many DNA molecules with a radioactive label on ...
[58] [59] High-throughput sequencing is intended to lower the cost of DNA sequencing beyond what is possible with standard dye-terminator methods. In ultra-high-throughput sequencing, as many as 500,000 sequencing-by-synthesis operations may be run in parallel. [60] [61] Illumina Genome Analyzer II System.
The first DNA sequencing methods were developed by Gilbert (1973) [8] and Sanger (1975). [9] Gilbert introduced a sequencing method based on chemical modification of DNA followed by cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased ...
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