Search results
Results from the WOW.Com Content Network
This method is a faster process than dry heat sterilization. Steam sterilization is performed using an autoclave , sometimes called a converter or steam sterilizer. The object or liquid is placed in the autoclave chamber, which is then sealed and heated using pressurized steam to a temperature set point for a defined period of time.
Moist heat sterilization processes sterilize using hot air that is heavily laden with water vapor, which plays the most important role in the sterilization. [ 1 ] [ 2 ] Boiling a sample for 30 minutes or more will kill virtually all vegetative cells present, but will not kill spores , which can germinate shortly thereafter and resume growth.
Heat fixation is used for the fixation of single cell organisms, most commonly bacteria and archaea. The organisms are typically mixed with water or physiological saline which helps to evenly spread out the sample. Once diluted, the sample is spread onto a microscope slide. This diluted bacteria sample is commonly referred to as a smear after ...
The heat used during the UHT process can cause Maillard browning and change the taste and smell of dairy products. [5] An alternative process is flash pasteurization, in which the milk is heated to 72 °C (162 °F) for at least fifteen seconds. UHT milk packaged in a sterile container has a typical unrefrigerated shelf life of six to nine ...
Prior to the development of more efficient methods, this stain was performed using the Wirtz method with heat fixation and counterstain. Through the use of malachite green and a diluted ratio of carbol fuchsin, fixing bacteria in osmic acid was a great way to ensure no blending of dyes.
Tyndallization is a process from the nineteenth century for sterilizing substances, usually food, named after its inventor John Tyndall, that can be used to kill heat-resistant endospores. Although now considered dated, it is still occasionally used. [citation needed]
Dry heat sterilization of an object is one of the earliest forms of sterilization practiced. It uses hot air that is either free from water vapor or has very little of it, where this moisture plays a minimal or no role in the process of sterilization.
where g is the number of degrees below the retort temperature on a simple heating curve at the end of the heating period, B B is the time in minutes from the beginning of the process to the end of the heating period, and f h is the time in minutes required for the straight-line portion of the heating curve plotted semilogarithmically on paper ...