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  2. Fixation (histology) - Wikipedia

    en.wikipedia.org/wiki/Fixation_(histology)

    Heat fixation is used for the fixation of single cell organisms, most commonly bacteria and archaea. The organisms are typically mixed with water or physiological saline which helps to evenly spread out the sample. Once diluted, the sample is spread onto a microscope slide. This diluted bacteria sample is commonly referred to as a smear after ...

  3. Moist heat sterilization - Wikipedia

    en.wikipedia.org/wiki/Moist_heat_sterilization

    Moist heat sterilization processes sterilize using hot air that is heavily laden with water vapor, which plays the most important role in the sterilization. [ 1 ] [ 2 ] Boiling a sample for 30 minutes or more will kill virtually all vegetative cells present, but will not kill spores , which can germinate shortly thereafter and resume growth.

  4. Sterilization (microbiology) - Wikipedia

    en.wikipedia.org/wiki/Sterilization_(microbiology)

    Microorganisms growing on an agar plate. Sterilization (British English: sterilisation) refers to any process that removes, kills, or deactivates all forms of life (particularly microorganisms such as fungi, bacteria, spores, and unicellular eukaryotic organisms) and other biological agents (such as prions or viruses) present in fluid or on a specific surface or object. [1]

  5. Tyndallization - Wikipedia

    en.wikipedia.org/wiki/Tyndallization

    Tyndallization is a process from the nineteenth century for sterilizing substances, usually food, named after its inventor John Tyndall, that can be used to kill heat-resistant endospores. Although now considered dated, it is still occasionally used. [citation needed]

  6. Microbiology - Wikipedia

    en.wikipedia.org/wiki/Microbiology

    Microbiology (from Ancient Greek μῑκρος (mīkros) ' small ' βίος (bíos) ' life ' and -λογία ' study of ') is the scientific study of microorganisms, those being of unicellular (single-celled), multicellular (consisting of complex cells), or acellular (lacking cells).

  7. Negative stain - Wikipedia

    en.wikipedia.org/wiki/Negative_stain

    In the case of transmission electron microscopy, opaqueness to electrons is related to the atomic number, i.e., the number of protons.Some suitable negative stains include ammonium molybdate, uranyl acetate, uranyl formate, phosphotungstic acid, osmium tetroxide, osmium ferricyanide [clarification needed] [2] and auroglucothionate.

  8. Ultra-high-temperature processing - Wikipedia

    en.wikipedia.org/wiki/Ultra-high-temperature...

    Ultra-high temperature processing (UHT), ultra-heat treatment, or ultra-pasteurization [1] is a food processing technology that sterilizes liquid food by heating it above 140 °C (284 °F) – the temperature required to kill bacterial endospores – for two to five seconds. [2]

  9. Z-value (temperature) - Wikipedia

    en.wikipedia.org/wiki/Z-value_(temperature)

    "F 0" is defined as the number of equivalent minutes of steam sterilization at temperature 121.1 °C (250 °F) delivered to a container or unit of product calculated using a z-value of 10 °C.