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An assay (analysis) is never an isolated process, as it must be accompanied with pre- and post-analytic procedures. Both the communication order (the request to perform an assay plus related information) and the handling of the specimen itself (the collecting, documenting, transporting, and processing done before beginning the assay) are pre-analytic steps.
Dry basis (also d.b., dry matter basis, DM) is an expression of a calculation in chemistry, chemical engineering and related subjects, in which the presence of water ...
The plaque reduction neutralization test is used to quantify the titer of neutralizing antibody for a virus. [1] [2]The serum sample or solution of antibody to be tested is diluted and mixed with a viral suspension.
If the measured response is binary, the assay is quantal; if not, it is quantitative. [3] A bioassay may be used to detect biological hazards or to give an assessment of the quality of a mixture. [4] A bioassay is often used to monitor water quality as well as wastewater discharges and its impact on the surroundings. [5]
Loop-mediated isothermal amplification (LAMP) primers [1] Loop-mediated isothermal amplification (LAMP) product [1]. In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140–149 °F) using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication activity.
Protein purification is a critical process in molecular biology and biochemistry, aimed at isolating a specific protein from a complex mixture, such as cell lysates or tissue extracts. [9] The goal is to obtain the protein in a pure form that retains its biological activity for further study, including functional assays, structural analysis, or ...
Wet chemistry commonly uses laboratory glassware such as beakers and graduated cylinders to prevent materials from being contaminated or interfered with by unintended sources. [3] Gasoline, Bunsen burners , and crucibles may also be used to evaporate and isolate substances in their dry forms.
It is less perturbative because the sample is not dried onto a surface, this drying process is often done in negative-stain TEM, and because Cryo-EM does not require contrast agent like heavy metal salts (e.g. uranyl acetate or phoshotungstic acid) which also may affect the structure of the biomolecule. Transmission electron microscopy, as a ...
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