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An assay (analysis) is never an isolated process, as it must be accompanied with pre- and post-analytic procedures. Both the communication order (the request to perform an assay plus related information) and the handling of the specimen itself (the collecting, documenting, transporting, and processing done before beginning the assay) are pre-analytic steps.
On the wet basis the value is the ratio of the weight of water to the total weight of the solution (1 / 5 = 20% in the example), so the moisture content is always below 100% (in the previous examples the moisture content was specified on this "moisture content wet basis"). For the moisture content dry basis the ratio of the weight of the water ...
If the measured response is binary, the assay is quantal; if not, it is quantitative. [3] A bioassay may be used to detect biological hazards or to give an assessment of the quality of a mixture. [4] A bioassay is often used to monitor water quality as well as wastewater discharges and its impact on the surroundings. [5]
Biological specimens in an elementary school science lab.. A biological specimen (also called a biospecimen) is a biological laboratory specimen held by a biorepository for research.
Loop-mediated isothermal amplification (LAMP) primers [1] Loop-mediated isothermal amplification (LAMP) product [1]. In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140–149 °F) using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication activity.
The purpose of this technique is to analyze the activity of a gene transcription promoter (in terms of expression of a so-called reporter gene under the regulatory control of that promoter) either in a quantitative manner, involving some measure of activity, or qualitatively (on versus off) through visualization of its activity in different cells, tissues, or organs.
C 0 t analysis, a technique based on the principles of DNA reassociation kinetics, is a biochemical technique that measures how much repetitive DNA is in a DNA sample such as a genome. [1]
The assay is well tolerant to diverse chemistries, as exemplified by the development of a nuclease assay for morpholino oligonucleotides. [9] This assay set-up can lack robustness and is not suitable for validation following the FDA's guidelines for bioanalytical method validation. This is demonstrated by an absence of published method that ...