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In 90% acetone-water, the peak absorption wavelengths of chlorophyll a are 430 nm and 664 nm; peaks for chlorophyll b are 460 nm and 647 nm; peaks for chlorophyll c 1 are 442 nm and 630 nm; peaks for chlorophyll c 2 are 444 nm and 630 nm; peaks for chlorophyll d are 401 nm, 455 nm and 696 nm. [27]
Chlorophyll a absorbs light within the violet, blue and red wavelengths. Accessory photosynthetic pigments broaden the spectrum of light absorbed, increasing the range of wavelengths that can be used in photosynthesis. [5] The addition of chlorophyll b next to chlorophyll a extends the absorption spectrum.
The infrared spectrum of liquid water is dominated by the intense absorption due to the fundamental O-H stretching vibrations. Because of the high intensity, very short path lengths, usually less than 50 μm, are needed to record the spectra of aqueous solutions.
The refractive index of water at 20 °C for visible light is 1.33. [1] The refractive index of normal ice is 1.31 (from List of refractive indices).In general, an index of refraction is a complex number with real and imaginary parts, where the latter indicates the strength of absorption loss at a particular wavelength.
The use of both together enhances the size of the absorption of light for producing energy. Chlorophyll b is a form of chlorophyll. Chlorophyll b helps in photosynthesis by absorbing light energy. It is more soluble than chlorophyll a in polar solvents because of its carbonyl group. Its color is green, and it primarily absorbs blue light. [2]
Top: Absorption spectra for chlorophyll-A, chlorophyll-B, and carotenoids extracted in a solution. Bottom: PAR action spectrum (oxygen evolution per incident photon) of an isolated chloroplast. Chlorophyll, the most abundant plant pigment, is most efficient in capturing red and blue light.
Absorbance spectra of free chlorophyll a (blue) and b (red) in a solvent. The action spectra of chlorophyll molecules are slightly modified in vivo depending on specific pigment-protein interactions. An action spectrum is a graph of the rate of biological effectiveness plotted against wavelength of light. [1]
The nature of the central metal can also influence the absorption spectrum of the metal-macrocycle complex or properties such as excited state lifetime. [ 4 ] [ 5 ] [ 6 ] The tetrapyrrole moiety in organic compounds which is not macrocyclic but still has a conjugated pi-bond system still acts as a chromophore.