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The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
Agar plates may also be indicator plates, in which the organisms are not selected based on growth, but are instead distinguished by a color change in some colonies, typically caused by the action of an enzyme on some compound added to the medium. [6] The plates are incubated for 12 hours up to several days, depending on the test that is performed.
After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars). Bismuth sulfite agar is a type of agar media used to isolate Salmonella species.
LB medium bottle and LB agar plate Plate medium agar LB. Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani, intended LB to stand for lysogeny broth, [1] but LB has also come to colloquially mean Luria broth, Lennox broth, life broth or Luria–Bertani medium. [2]
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
The plates are left upright on the bench to dry before inversion and incubation at 37 °C for 18 – 24 hours (or appropriate incubation conditions considering the organism and agar used). Each sector is observed for growth, high concentrations will give a confluent growth over the area of the drop, or a large number of small/merged colonies.
The Wilhelmy plate consists of a thin plate usually on the order of a few square centimeters in area. The plate is often made from filter paper, glass or platinum which may be roughened to ensure complete wetting. In fact, the results of the experiment do not depend on the material used, as long as the material is wetted by the liquid. [1]
YPD medium bottle and YPD agar plate. YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose). [1]