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Bromothymol blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). A common use is for measuring the presence of carbonic acid in a liquid. It is typically sold in solid form as the sodium salt of the acid ...
The increase in pH then causes color change in the bromothymol blue indicator, turning it blue. Under neutral conditions the medium remains a green color. The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change.
yellow 0.0 2.0 blue-violet Malachite green (first transition) yellow 0.0 2.0 green Malachite green (second transition) green 11.6 14.0 colorless Thymol blue (first transition) red 1.2 2.8 yellow Thymol blue (second transition) yellow 8.0 9.6 blue Methyl yellow: red 2.9 4.0 yellow Methylene blue: colorless 5.0 9.0 dark blue Bromophenol blue ...
Sucrose fermentation produces acid, which converts the colour of bromothymol blue or thymol blue. Two dyes rather than one make the medium produce an array of yellow, green, or blue so that differentiating among various Vibrio species is possible.
It seems as though I have entered into an intentioned editing battle over the pKa of bromothymol blue. I changed the pKa value from 7.10 to 7.0 since the CRC of Handbook of Chemistry and Physics - 86th ed. (2005-2006) lists the pKa as 7.0.
Rather than becoming unsafe, they lose potency slowly over time.” If they’re stored in optimal conditions, you can get the most bang for your buck. Best case scenario, the average shelf-life ...
It transitions from red to yellow at pH 1.2–2.8 and from yellow to blue at pH 8.0–9.6. It is usually a component of Universal indicator. At wavelength (378 - 382) nm, extinction coefficient > 8000 and at wavelength (298 - 302) nm, the extinction coefficient > 12000. [3]
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