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Used to automate the DNA sequencing process. Genetics, Molecular biology. Enzyme-linked immunosorbent assay (ELISA) Used to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measure. Biochemistry, Molecular biology. Gene knockout.
t. e. Molecular biology / məˈlɛkjʊlər / is a branch of biology that seeks to understand the molecular basis of biological activity in and between cells, including biomolecular synthesis, modification, mechanisms, and interactions. [1][2][3] Though cells and other microscopic structures had been observed in living organisms as early as the ...
RT-PCR. Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [1] It is primarily used to measure the amount of a specific RNA.
Molecular diagnostics. Molecular diagnostics is a collection of techniques used to analyze biological markers in the genome and proteome, and how their cells express their genes as proteins, applying molecular biology to medical testing. In medicine the technique is used to diagnose and monitor disease, detect risk, and decide which therapies ...
Bioanalysis. Bioanalysis is a sub-discipline of analytical chemistry covering the quantitative measurement of xenobiotics (drugs and their metabolites, and biological molecules in unnatural locations or concentrations) and biotics (macromolecules, proteins, DNA, large molecule drugs, metabolites) in biological systems.
Microbial cultures on solid and liquid media. A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology.
The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins [1] by their ability to bind to specific oligonucleotide probes. Determination of molecular weight of proteins binding to DNA is also made possible by the technique. The name originates from a combination of ideas underlying ...
In vitro (Latin for "in glass"; often not italicized in English usage [3][4][5]) studies are conducted using components of an organism that have been isolated from their usual biological surroundings, such as microorganisms, cells, or biological molecules. For example, microorganisms or cells can be studied in artificial culture media, and ...