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An endospore stain of the cell Bacillus subtilis showing endospores as green and the vegetative cell as red Phase-bright endospores of Paenibacillus alvei imaged with phase-contrast microscopy. An endospore is a dormant, tough, and non-reproductive structure produced by some bacteria in the phylum Bacillota.
Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1] Within bacteria, endospores are protective structures used to survive extreme conditions, including high temperatures making them highly resistant to chemicals. [ 2 ]
Bacillus species are rod-shaped, endospore-forming aerobic or facultatively anaerobic, Gram-positive bacteria; in some species cultures may turn Gram-negative with age. The many species of the genus exhibit a wide range of physiologic abilities that allow them to live in every natural environment.
As with other members of the genus Bacillus, it can form an endospore, to survive extreme environmental conditions of temperature and desiccation. [12] B. subtilis is a facultative anaerobe [6] [13] and had been considered as an obligate aerobe until 1998. B. subtilis is heavily flagellated, which gives it the ability to move quickly in liquids.
Bacillus cereus is a Gram-positive rod-shaped bacterium commonly found in soil, food, and marine sponges. [1] The specific name, ... Bacillus cereus endospore stain.
Fig1. The sporulation process of Bacillus subtilis. Bacillus subtilis is a rod-shaped, Gram-positive bacteria that is naturally found in soil and vegetation, and is known for its ability to form a small, tough, protective and metabolically dormant endospore.
Bacillus anthracis is a gram-positive and rod-shaped bacterium that causes anthrax, a deadly disease to livestock and, occasionally, to humans. It is the only permanent ( obligate ) pathogen within the genus Bacillus .
A stained preparation of Bacillus subtilis showing endospores as green and the vegetative cell as red. The Schaeffer–Fulton stain is a technique designed to isolate endospores by staining any present endospores green, and any other bacterial bodies red. [1]