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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
Longer read lengths of each single electrophoretic separation, substantially reduces the cost associated with de novo DNA sequencing and the number of templates needed to sequence DNA contigs at a given redundancy. Microfluidics may allow for faster, cheaper and easier sequence assembly. [16]
In the mathematical discipline of graph theory, a graph labeling is the assignment of labels, traditionally represented by integers, to edges and/or vertices of a graph. [1] Formally, given a graph G = (V, E), a vertex labeling is a function of V to a set of labels; a graph with such a function defined is called a vertex-labeled graph.
Pyrosequencing uses luciferase to generate light for detection of the individual nucleotides added to the nascent DNA, and the combined data are used to generate sequence reads. [81] This technology provides intermediate read length and price per base compared to Sanger sequencing on one end and Solexa and SOLiD on the other.
View of the start of an example dye-terminator read (click to expand) An alternative to the labelling of the primer is to label the terminators instead, commonly called 'dye terminator sequencing'. The major advantage of this approach is the complete sequencing set can be performed in a single reaction, rather than the four needed with the ...
A graceful labeling. Vertex labels are in black, edge labels in red.. In graph theory, a graceful labeling of a graph with m edges is a labeling of its vertices with some subset of the integers from 0 to m inclusive, such that no two vertices share a label, and each edge is uniquely identified by the absolute difference between its endpoints, such that this magnitude lies between 1 and m ...
Pyrosequencing, a new method of DNA sequencing which involves the addition of phosphorylated dNTP's to the DNA polymerase reaction which emmit light upon binding to the DNA template. It makes DNA sequencing significantly faster than with the current chain termination method. We do use them, look at Pyrosequencing/Temp.