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Virus crystallisation is the re-arrangement of viral components into solid crystal particles. [1] The crystals are composed of thousands of inactive forms of a particular virus arranged in the shape of a prism. [2] The inactive nature of virus crystals provide advantages for immunologists to effectively analyze the structure and function behind ...
Crystallization; Fundamentals; Crystal; Crystal structure; Nucleation; Concepts; Crystallization; Crystal growth; Recrystallization; Seed crystal; Protocrystalline
By providing information on mechanism of action, epitope mapping is a critical component in therapeutic monoclonal antibody (mAb) development. Epitope mapping can reveal how a mAb exerts its functional effects - for instance, by blocking the binding of a ligand or by trapping a protein in a non-functional state.
The viral eukaryogenesis hypothesis posits that eukaryotes are composed of three ancestral elements: a viral component that became the modern nucleus; a prokaryotic cell (an archaeon according to the eocyte hypothesis) which donated the cytoplasm and cell membrane of modern cells; and another prokaryotic cell (here bacterium) that, by endocytosis, became the modern mitochondrion or chloroplast.
The sizes of viruses determined using this new microscope fitted in well with those estimated by filtration experiments. Viruses were expected to be small, but the range of sizes came as a surprise. Some were only a little smaller than the smallest known bacteria, and the smaller viruses were of similar sizes to complex organic molecules. [14]
A virus with this "viral envelope" uses it—along with specific receptors—to enter a new host cell. Viruses vary in shape from the simple helical and icosahedral to more complex structures. Viruses range in size from 20 to 300 nanometres; it would take 33,000 to 500,000 of them, side by side, to stretch to 1 centimetre (0.4 in).
Crystallization is the process by which solids form, where the atoms or molecules are highly organized into a structure known as a crystal. Some ways by which crystals form are precipitating from a solution , freezing , or more rarely deposition directly from a gas .
The solvents vaporize, leaving only the recrystallized matrix, but now with analyte molecules embedded into MALDI crystals. The matrix and the analyte are said to be co-crystallized. Co-crystallization is a key issue in selecting a proper matrix to obtain a good quality mass spectrum of the analyte of interest.