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The simplest way to prepare a BBS solution is to use BBS tablets. They are formulated to give a ready to use borate buffered saline solution upon dissolution in 500 ml of deionized water. Concentration of borate and NaCl as well as the pH can vary, and the resulting solution would still be referred to as "borate buffered saline".
Common borate salts include sodium metaborate (NaBO 2) and borax. Borax is soluble in water, so mineral deposits only occur in places with very low rainfall. Extensive deposits were found in Death Valley and shipped with twenty-mule teams from 1883 to 1889. In 1925, deposits were found at Boron, California on the edge of the Mojave Desert.
Borax (also referred to as sodium borate, tincal (/ ˈ t ɪ ŋ k əl /) and tincar (/ ˈ t ɪ ŋ k ər /)) is a salt (ionic compound), a hydrated or anhydrous borate of sodium, with the chemical formula Na 2 H 20 B 4 O 17. [1] [a] It is a colorless crystalline solid that dissolves in water to make a basic solution.
The hydrated tetraborate anion occurs in the mineral borax (sodium tetraborate octahydrate) with the formula Na 2 [B 4 O 5 (OH) 4]·8H 2 O. The borax chemical formula is also commonly written in a more compact notation as Na 2 B 4 O 7 ·10H 2 O. Sodium borate can be obtained in high purity and so can be used to make a standard solution in ...
The Borate Minerals are minerals which contain a borate anion group. The borate (BO 3) units may be polymerised similar to the SiO 4 unit of the silicate mineral class. This results in B 2 O 5, B 3 O 6, B 2 O 4 anions as well as more complex structures which include hydroxide or halogen anions. [2] The [B(O,OH) 4] − anion exists as well.
Downstream applications, such as isolation of DNA from a gel slice or southern blot analysis, work as expected with sodium borate gels. LB buffer containing lithium borate is similar to sodium borate and has all of its advantages, but permits use of even higher voltages due to the lower conductivity of lithium ions as compared to sodium ions. [1]
LB buffer, also known as lithium borate buffer, is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. It is made up of Lithium borate ( lithium hydroxide monohydrate and boric acid ).
The solution of orthoboric acid and borax in 4:5 ratio is used as a fire retarding agent of wood by impregnation. [38] It is also used in the manufacturing of ramming mass, a fine silica-containing powder used for producing induction furnace linings and ceramics. Boric acid is added to borax for use as welding flux by blacksmiths. [39]