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2-Phenylethyl bromide is an organobromide with the formula C 6 H 5 CH 2 CH 2 Br. It is a colorless liquid, although older samples appear yellow. Analogous to the preparation of most 1-bromoalkanes, it is prepared by free-radical addition of hydrogen bromide to styrene. These conditions lead to anti-Markovnikov addition, giving the 1-bromo ...
Increasing ethidium bromide intercalated into the DNA can change it from a negatively supercoiled molecule into a fully relaxed form, then to positively coiled superhelix at maximum intercalation. [9] Agarose gel electrophoresis can be used to resolve circular DNA with different supercoiling topology.
Silver bromide (AgBr). Nearly all elements in the periodic table form binary bromides. The exceptions are decidedly in the minority and stem in each case from one of three causes: extreme inertness and reluctance to participate in chemical reactions (the noble gases, with the exception of xenon in the very unstable XeBr 2; extreme nuclear instability hampering chemical investigation before ...
In organic chemistry, the bromine test is a qualitative test for the presence of unsaturation (carbon-to-carbon double or triple bonds), phenols and anilines. An unknown sample is treated with a small amount of elemental bromine in an organic solvent, being as dichloromethane or carbon tetrachloride. Presence of unsaturation and/or phenol or ...
A number of factors can affect the migration of nucleic acids: the dimension of the gel pores (gel concentration), size of DNA being electrophoresed, the voltage used, the ionic strength of the buffer, and the concentration of intercalating dye such as ethidium bromide if used during electrophoresis. [11]
Close-up of DNA ladders on an agarose gel. GelRed stain was used. Loading of a sample into a polyacrylamide gel electrophoresis well. An electrophoretic color marker is a chemical used to monitor the progress of agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE) since DNA, RNA, and most proteins are colourless. [1]
Gel conditions are 1% agarose, 3 volt/cm, and ethidium bromide stain. A molecular-weight size marker , also referred to as a protein ladder , DNA ladder , or RNA ladder , is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis , using the principle that molecular weight is inversely ...
Reference ranges (reference intervals) for blood tests are sets of values used by a health professional to interpret a set of medical test results from blood samples. Reference ranges for blood tests are studied within the field of clinical chemistry (also known as "clinical biochemistry", "chemical pathology" or "pure blood chemistry"), the ...