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  2. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    HPLC is distinguished from traditional ("low pressure") liquid chromatography because operational pressures are significantly higher (around 50–1400 bar), while ordinary liquid chromatography typically relies on the force of gravity to pass the mobile phase through the packed column. Due to the small sample amount separated in analytical HPLC ...

  3. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high-performance liquid chromatography. In HPLC the sample is forced by a liquid at high pressure (the mobile phase) through a column that is packed with a stationary phase composed of irregularly or ...

  4. Liquid chromatography–mass spectrometry - Wikipedia

    en.wikipedia.org/wiki/Liquid_chromatography...

    The liquid solvent (mobile phase) is delivered under high pressure (up to 400 bar or 5800 psi) into a packed column containing the stationary phase. The high pressure is necessary to achieve a constant flow rate for reproducible chromatography experiments.

  5. Atmospheric-pressure chemical ionization - Wikipedia

    en.wikipedia.org/wiki/Atmospheric-pressure...

    Atmospheric pressure chemical ionization chamber cross section. Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry which utilizes gas-phase ion-molecule reactions at atmospheric pressure (10 5 Pa), [1] [2] commonly coupled with high-performance liquid chromatography (HPLC). [3]

  6. Capillary electrochromatography - Wikipedia

    en.wikipedia.org/.../Capillary_electrochromatography

    Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube, which is filled with the analyte. High-performance liquid chromatography separates analytes by passing them, under high pressure, through a column filled with stationary phase. The ...

  7. Monolithic HPLC column - Wikipedia

    en.wikipedia.org/wiki/Monolithic_HPLC_column

    In order to withstand the pressure, a new field of chromatography came into being: UHPLC or UPLC- ultra high pressure liquid chromatography. The new instruments were able to endure pressures of up to 15,000 pounds per square inch (1,000 bar), as opposed to conventional machines, which, as previously state, can hold up to 5,000 pounds per square ...

  8. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...

  9. Chromatography detector - Wikipedia

    en.wikipedia.org/wiki/Chromatography_detector

    A chromatography detector is a device that detects and quantifies separated compounds as they elute from the chromatographic column.These detectors are integral to various chromatographic techniques, such as gas chromatography, [1] liquid chromatography, and high-performance liquid chromatography, [2] and supercritical fluid chromatography [3] among others.

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