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The spot indole test is used to determine if a microbe can deaminate tryptophan to produce indole. This test is performed by saturating a piece of filter paper with Indole Kovacs Reagent and scraping a portion of microbe onto the paper. A color to a pink-red color indicates a positive result while no color change indicates the lack of ...
an organic compound; primary use is to couple amino acids during artificial peptide synthesis Diethyl azodicarboxylate: a valuable reagent but also quite dangerous and explodes upon heating Diethyl ether: organic compound; a common laboratory solvent Dihydropyran: a heterocyclic compound; used as a protecting group for alcohols in organic ...
A bioassay is a biochemical test to estimate the potency of a sample compound. Usually this potency can only be measured relative to a standard compound. [3] [1] A typical bioassay involves a stimulus (ex. drugs) applied to a subject (ex. animals, tissues, plants). The corresponding response (ex. death) of the subject is thereby triggered and ...
A spot assay or spot test can also refer to a specific test in microbiology. This test is often used to check the growth rate of bacterial or yeast cells on different media or to perform serial dilution tests of micro-organisms. Usually a 96-pinner (often called frogger) is used to perform these spot assay.
The laboratory procedure involves making serial dilutions of the sample (1:10, 1:100, 1:1000, etc.) in sterile water and cultivating these on nutrient agar in a dish that is sealed and incubated. Typical media include plate count agar for a general count or MacConkey agar to count Gram-negative bacteria such as E. coli. Typically one set of ...
The analyte can be a drug, biochemical substance, chemical element or compound, or cell in an organism or organic sample. [ 1 ] [ 2 ] An assay usually aims to measure an analyte's intensive property and express it in the relevant measurement unit (e.g. molarity , density , functional activity in enzyme international units, degree of effect in ...
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