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A sputum culture is a test to detect and identify bacteria [1] or fungi that infect the lungs or breathing passages. Sputum is a thick fluid produced in the lungs and in the adjacent airways. Normally, fresh morning sample is preferred for the bacteriological examination of sputum. [ 2 ]
The preferred method for this is fluorescence microscopy (auramine-rhodamine staining), which is more sensitive than conventional Ziehl–Neelsen staining. [4] In cases where there is no spontaneous sputum production, a sample can be induced, usually by inhalation of a nebulized saline or saline with bronchodilator solution.
Whitish gray sputum color against a white color background (such as a white sink surface) tends to indicate either a specimen from someone who is dehydrated, and/or from an older person, and/or a specimen with a mixed, modest number of eosinophils and maybe some acute inflammatory neutrophil cells (this last choice tends to suggest chronic ...
Sampling of sputum from the lungs for sputum culture. It can be performed by special techniques of coughing, or by a protected specimen brush (PSB), [ 1 ] which is a brush that can be retracted into a plastic tube to prevent contamination of bacteria in the throat while inserting and removing the instrument.
Bronchoalveolar lavage (BAL), also known as bronchoalveolar washing, is a diagnostic method of the lower respiratory system in which a bronchoscope is passed through the mouth or nose into an appropriate airway in the lungs, with a measured amount of fluid introduced and then collected for examination.
Burkholderia cepacia complex Scientific classification Domain: Bacteria Phylum: Pseudomonadota Class: Betaproteobacteria Order: Burkholderiales Family: Burkholderiaceae Genus: Burkholderia Species complex: B. cepacia complex Burkholderia cepacia complex (BCC) is a species complex consisting of Burkholderia cepacia and at least 20 different biochemically similar species of Gram-negative ...
Specimens for antibiotic sensitivity testing are ideally collected before treatment is started. [1] A sample may be taken from the site of a suspected infection; such as a blood culture sample when bacteria are suspected to be present in the bloodstream ( bacteraemia ), a sputum sample in the case of a pneumonia , or a urine sample in the case ...
To collect the sample, the swab is inserted in the nostril and gently moved forward into the nasopharynx, a region of the pharynx that covers the roof of the mouth. [9] The swab is then rotated for a specified period of time to collect secretions, then the swab is removed and placed into a sterile viral transport media , which preserves the ...