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Bromothymol blue is synthesized by addition of elemental bromine to thymol blue in a solution in glacial acetic acid. [ 6 ] To prepare a solution for use as pH indicator, dissolve 0.10 g in 8.0 cm 3 N/50 (a.k.a. 0.02 Normal ) NaOH and dilute with water to 250 cm 3 .
Solution: The main components of a universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue, and phenolphthalein. This mixture is important because each component loses or gains protons depending upon the acidity or alkalinity of the solution being tested. It is beneficial to use this type of universal ...
Chemical structure of phenolphthalein, a common phthalein dye. Phthalein dyes are a class of dyes mainly used as pH indicators, due to their ability to change colors depending on pH. [1]
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The increase in pH then causes color change in the bromothymol blue indicator, turning it blue. Under neutral conditions the medium remains a green color. The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change.
The standards used include potassium dichromate (isosbestic points at 339 and 445 nm), bromothymol blue (325 and 498 nm) and congo red (541 nm). The wavelength of the isosbestic point determined does not depend on the concentration of the substance used, and so it becomes a very reliable reference.
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