Search results
Results from the WOW.Com Content Network
The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated. [1] Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full ...
Progress of replication forks is inhibited by many factors; collision with proteins or with complexes binding strongly on DNA, deficiency of dNTPs, nicks on template DNAs and so on. If replication forks get stuck and the rest of the sequences from the stuck forks are not copied, then the daughter strands get nick nick unreplicated sites.
Reproducibility, closely related to replicability and repeatability, is a major principle underpinning the scientific method.For the findings of a study to be reproducible means that results obtained by an experiment or an observational study or in a statistical analysis of a data set should be achieved again with a high degree of reliability when the study is replicated.
Six different proteins of the AAA+ ATPase family that form a hexamer in solution. This hexamer is recruited and loaded by ORC, Cdc6 and Cdt1 and forms a double hexamer that is topologically linked around DNA to form a salt-resistant pre-replicative complex. On replication initiation, Mcm2-7 moves away from ORC with replication fork. Mcm10
Used to rapidly make millions to billions of copies (complete copies or partial copies) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail
Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...
Cdt1 binding and the ATPase activity of ORC and Cdc6 facilitate the loading of the minichromosome maintenance (MCM) proteins 2-7 onto the chromatin. [1] The MCM complex is the DNA helicase that opens the helix at the replication origin and unwinds the two strands as the replication forks travel along the DNA. [5]
Replication (scientific method), one of the main principles of the scientific method, a.k.a. reproducibility Replication (statistics), the repetition of a test or complete experiment; Replication crisis; Self-replication, the process in which an entity (a cell, virus, program, etc.) makes a copy of itself