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NS1 antigen test (nonstructural protein 1) is a test for dengue, introduced in 2006. It allows rapid detection on the first day of fever, before antibodies appear some 5 or more days later. [1] It has been adopted for use in some 40 nations. The method of detection is through enzyme-linked immunosorbent assay.
The test was once part of the World Health Organization (WHO) algorithm for diagnosis of dengue fever, [3] however it is no longer used in the latest WHO guidance. [4] Studies have shown that the tourniquet test has low predictive value for dengue fever and should be used in conjunction with other tests for a reliable diagnosis. [5] [6] [7] [8]
The plaque reduction neutralization test is used to quantify the titer of neutralizing antibody for a virus. [1] [2] The serum sample or solution of antibody to be tested is diluted and mixed with a viral suspension. This is incubated to allow the antibody to react with the virus. This is poured over a confluent monolayer of host cells.
Under the new advisory, doctors are advised to be aware of the increased possibility of dengue among patients, and to consider issuing dengue tests if necessary. Is dengue fever common in the U.S.?
Dengue virus (DENV) is the cause of dengue fever.It is a mosquito-borne, single positive-stranded RNA virus of the family Flaviviridae; genus Flavivirus. [1] [2] Four serotypes of the virus have been found, and a reported fifth has yet to be confirmed, [3] [4] [5] all of which can cause the full spectrum of disease. [1]
Graph of when laboratory tests for dengue fever become positive. Day zero refers to the start of symptoms, 1st refers to in those with a primary infection, and 2nd refers to in those with a secondary infection. [21] Warning symptoms of severe dengue include abdominal pain, persistent vomiting, odema, bleeding, lethargy, and liver enlargement.
When laboratory tests for dengue fever become positive where day zero is the start of symptoms, 1st refers to in those with a primary infection, and 2nd refers to in those with a secondary infection. [7] Dengue fever may be diagnosed by microbiological laboratory testing. [26]
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