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In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...
Selectable markers allow scientists to separate non-recombinant organisms (those which do not contain the selectable marker) from recombinant organisms (those which do); that is, a recombinant DNA molecule such as a plasmid expression vector is introduced into bacterial cells, and some bacteria are successfully transformed while some remain non-transformed.
Next to the above-mentioned selection makers a few auxotrophic strains were generated to work with auxotrophic makers. The URA3 marker (URA3 blaster method) is an often-used strategy in uridine auxotrophic strains; however, studies have shown that differences in URA3 position in the genome can be involved in the pathogeny of C. albicans. [119]
This is a list of roots, suffixes, and prefixes used in medical terminology, their meanings, and their etymologies. Most of them are combining forms in Neo-Latin and hence international scientific vocabulary. There are a few general rules about how they combine.
The Y-STR markers in the following list are commonly used in forensic [1] and genealogical DNA testing. DYS454 is the least diverse, and multi-copy marker DYS464 is the most diverse Y-STR marker. The location on the Y-chromosome of numbered Y-STR markers can be roughly given with cytogenetic localization. For example, DYS449 is located at Yp11 ...
Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces cerevisiae, which is then ligated into a bacterial plasmid. By inserting large fragments of DNA, from 100–1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking .
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A diagram of a how a reporter gene is used to study a regulatory sequence. In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants.