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Bromothymol blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). A common use is for measuring the presence of carbonic acid in a liquid. It is typically sold in solid form as the sodium salt of the acid ...
Bromothymol blue is the indicator used in the agar, it changes to yellow in case of acid production during fermentation of lactose or changes to deep blue in case of alkalinization. Lactose-positive bacteria build yellow colonies. Bacteria which decarboxylate L-cystine cause an alkaline reaction and build deep blue colonies. [1]
The increase in pH then causes color change in the bromothymol blue indicator, turning it blue. Under neutral conditions the medium remains a green color. The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change.
If your diarrhea lasts longer than a couple of days, Dr. Ahmad suggests calling your doctor, especially if you also have a fever, dehydration, bloody stool, weight loss, pain, and severe pain.
Some of the most common ones include abdominal pain and frequent diarrhea (loose or watery stool), constipation (hard or lumpy stool) or a mixture of both, explains Dr. William Chey, a physician ...
H + + Bromothymol blue (Blue) → Bromothymol blue-H + (Yellow) Elevated protein concentrations produce slightly elevated specific density results as a consequence of the indicator's protein error; in addition, samples with a pH above 6.5 give lower readings as a result of the indicator's bias.
Solution: The main components of a universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue, and phenolphthalein. This mixture is important because each component loses or gains protons depending upon the acidity or alkalinity of the solution being tested. It is beneficial to use this type of universal ...
It transitions from red to yellow at pH 1.2–2.8 and from yellow to blue at pH 8.0–9.6. It is usually a component of Universal indicator. At wavelength (378 - 382) nm, extinction coefficient > 8000 and at wavelength (298 - 302) nm, the extinction coefficient > 12000. [3]